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{"id":44030,"date":"2019-10-13T11:58:07","date_gmt":"2019-10-13T04:58:07","guid":{"rendered":"https:\/\/lop12.edu.vn\/?p=44030"},"modified":"2019-10-13T11:58:08","modified_gmt":"2019-10-13T04:58:08","slug":"ly-thuyet-sinh-hoc-10-bai-15-thuc-hanh-mot-so-thi-nghiem-ve-enzim","status":"publish","type":"post","link":"https:\/\/lop12.edu.vn\/ly-thuyet-sinh-hoc-10-bai-15-thuc-hanh-mot-so-thi-nghiem-ve-enzim\/","title":{"rendered":"L\u00fd thuy\u1ebft Sinh h\u1ecdc 10 B\u00e0i 15: Th\u1ef1c h\u00e0nh: M\u1ed9t s\u1ed1 th\u00ed nghi\u1ec7m v\u1ec1 enzim"},"content":{"rendered":"\n

L\u00fd thuy\u1ebft Sinh h\u1ecdc 10 B\u00e0i 15: Th\u1ef1c h\u00e0nh: M\u1ed9t s\u1ed1 th\u00ed nghi\u1ec7m v\u1ec1 enzim<\/h2>\n\n\n\n

I. Th\u00ed nghi\u1ec7m v\u1edbi enzim Catalaza<\/h3>\n\n\n\n

1. M\u1ee5c ti\u00eau <\/strong> <\/p>\n\n\n\n

– Bi\u1ebft c\u00e1ch b\u1ed1 tr\u00ed th\u00ed nghi\u1ec7m v\u00e0 t\u1ef1 \u0111\u00e1nh gi\u00e1 \u0111\u01b0\u1ee3c m\u1ee9c \u0111\u1ed9 \u1ea3nh h\u01b0\u1edfng c\u1ee7a c\u00e1c y\u1ebfu t\u1ed1 m\u00f4i tr\u01b0\u1eddng l\u00ean h\u1ecdt t\u00ednh c\u1ee7a enzim catalaza.<\/p>\n\n\n\n

2. Chu\u1ea9n b\u1ecb<\/strong> <\/p>\n\n\n\n

a. M\u1eabu v\u1eadt : \n<\/p>\n\n\n\n

– C\u1ee7 khoai t\u00e2y s\u1ed1ng, c\u1ee7 khoai t\u00e2y \u0111\u00e3 n\u1ea5u ch\u00edn.\n<\/p>\n\n\n\n

b. D\u1ee5ng c\u1ee5:\n<\/p>\n\n\n\n

– \u0110\u0129a petri, dao c\u1eaft, \u1ed1ng nh\u1ecf gi\u1ecdt.\n<\/p>\n\n\n\n

c. H\u00f3a ch\u1ea5t : \n<\/p>\n\n\n\n

– Dung d\u1ecbch H2O2\t\n<\/p>\n\n\n\n

– N\u01b0\u1edbc \u0111\u00e1.\t\t\n<\/p>\n\n\n\n

3. Ti\u1ebfn h\u00e0nh<\/strong> <\/p>\n\n\n\n

– B\u01b0\u1edbc 1: C\u1eaft khoai t\u00e2y s\u1ed1ng v\u00e0 khoai t\u00e2y ch\u00edn th\u00e0nh l\u00e1t m\u1ecfng\n<\/p>\n\n\n\n

– B\u01b0\u1edbc 2: Cho m\u1ed9t s\u1ed1 l\u00e1t khoai t\u00e2y s\u1ed1ng v\u00e0o trong khay \u0111\u1ef1ng n\u01b0\u1edbc \u0111\u00e1 \nho\u1eb7c trong ng\u0103n m\u00e1t t\u1ee7 l\u1ea1nh tr\u01b0\u1edbc khi th\u00ed nghi\u1ec7m kho\u1ea3ng 30 ph\u00fat.\n<\/p>\n\n\n\n

– B\u01b0\u1edbc 3: L\u1ea5y m\u1ed9t l\u00e1t khoai t\u00e2y s\u1ed1ng \u0111\u1ec3 \u1edf nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng th\u00ed nghi\u1ec7m,\n m\u1ed9t l\u00e1t \u0111\u00e3 lu\u1ed9c ch\u00edn v\u00e0 m\u1ed9t l\u00e1t khoai t\u00e2y s\u1ed1ng t\u1eeb t\u1ee7 l\u1ea1nh ra, r\u1ed3i d\u00f9ng \n\u1ed1ng h\u00fat nh\u1ecf l\u00ean gi\u1eefa m\u1ed7i l\u00e1t khoai t\u00e2y m\u1ed9t gi\u1ecdt H2O2.\n \n<\/p>\n\n\n\n

II. Th\u00ed nghi\u1ec7m s\u1eed d\u1ee5ng enzim trong qu\u1ea3 d\u1ee9a t\u01b0\u01a1i \u0111\u1ec3 t\u00e1ch chi\u1ebft ADN<\/h3>\n\n\n\n

1. M\u1ee5c ti\u00eau<\/strong> <\/p>\n\n\n\n

SGK<\/p>\n\n\n\n

2. Chu\u1ea9n b\u1ecb<\/strong> <\/p>\n\n\n\n

a, M\u1eabu v\u1eadt\n<\/p>\n\n\n\n

– D\u1ee9a t\u01b0\u01a1i\n<\/p>\n\n\n\n

– Gan g\u00e0 t\u01b0\u01a1i ho\u1eb7c gan l\u1ee3n\n<\/p>\n\n\n\n

b, D\u1ee5ng c\u1ee5 v\u00e0 ho\u00e1 ch\u1ea5t\n<\/p>\n\n\n\n

– \u1ed0ng nghi\u1ec7m, pipet, c\u1ed1c thu\u1ef7 tinh, m\u00e1y xay sinh t\u1ed1 (hay ch\u00e0y c\u1ed1i \nkh\u00e1c \u0111\u1ec3 nghi\u1ec1n m\u1eabu v\u1eadt), dao, th\u1edbt, ph\u1ec5u, v\u1ea3i m\u00e0n ho\u1eb7c l\u01b0\u1edbi l\u1ecdc, \u1ed1ng \n\u0111ong, que tre\n<\/p>\n\n\n\n

– C\u1ed3n \u00eatanol 70 – 900<\/sup>, n\u01b0\u1edbc l\u1ecdc l\u1ea1nh ho\u1eb7c n\u01b0\u1edbc c\u1ea5t l\u1ea1nh, ch\u1ea5t t\u1ea9y r\u1eeda.\n<\/p>\n\n\n\n

3. Ti\u1ebfn h\u00e0nh<\/strong> <\/p>\n\n\n\n

– B\u01b0\u1edbc 1: Nghi\u1ec1n m\u1eabu v\u1eadt\n<\/p>\n\n\n\n

– B\u01b0\u1edbc 2: T\u00e1ch ADN ra kh\u1ecfi t\u1ebf b\u00e0o v\u00e0 nh\u00e2n t\u1ebf b\u00e0o\n<\/p>\n\n\n\n

– B\u01b0\u1edbc 3: K\u1ebft t\u1ee7a ADN trong d\u1ecbch t\u1ebf b\u00e0o b\u1eb1ng c\u1ed3n\n<\/p>\n\n\n\n

– B\u01b0\u1edbc 4: T\u00e1ch ADN ra kh\u1ecfi l\u1edbp c\u1ed3n.\n<\/p>\n","protected":false},"excerpt":{"rendered":"

L\u00fd thuy\u1ebft Sinh h\u1ecdc 10 B\u00e0i 15: Th\u1ef1c h\u00e0nh: M\u1ed9t s\u1ed1 th\u00ed nghi\u1ec7m v\u1ec1 enzim I. Th\u00ed nghi\u1ec7m v\u1edbi enzim Catalaza 1. M\u1ee5c ti\u00eau – Bi\u1ebft c\u00e1ch b\u1ed1 tr\u00ed th\u00ed nghi\u1ec7m v\u00e0 t\u1ef1 \u0111\u00e1nh gi\u00e1 \u0111\u01b0\u1ee3c m\u1ee9c \u0111\u1ed9 \u1ea3nh h\u01b0\u1edfng c\u1ee7a c\u00e1c y\u1ebfu t\u1ed1 m\u00f4i tr\u01b0\u1eddng l\u00ean h\u1ecdt t\u00ednh c\u1ee7a enzim catalaza. 2. Chu\u1ea9n b\u1ecb […]<\/p>\n","protected":false},"author":12,"featured_media":44031,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_mi_skip_tracking":false,"tdm_status":"","tdm_grid_status":""},"categories":[1644],"tags":[],"yoast_head":"\nL\u00fd thuy\u1ebft Sinh h\u1ecdc 10 B\u00e0i 15: Th\u1ef1c h\u00e0nh: M\u1ed9t s\u1ed1 th\u00ed nghi\u1ec7m v\u1ec1 enzim<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/lop12.edu.vn\/ly-thuyet-sinh-hoc-10-bai-15-thuc-hanh-mot-so-thi-nghiem-ve-enzim\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"L\u00fd thuy\u1ebft Sinh h\u1ecdc 10 B\u00e0i 15: Th\u1ef1c h\u00e0nh: M\u1ed9t s\u1ed1 th\u00ed nghi\u1ec7m v\u1ec1 enzim\" \/>\n<meta property=\"og:description\" content=\"L\u00fd thuy\u1ebft Sinh h\u1ecdc 10 B\u00e0i 15: Th\u1ef1c h\u00e0nh: M\u1ed9t s\u1ed1 th\u00ed nghi\u1ec7m v\u1ec1 enzim I. 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Chu\u1ea9n b\u1ecb […]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/lop12.edu.vn\/ly-thuyet-sinh-hoc-10-bai-15-thuc-hanh-mot-so-thi-nghiem-ve-enzim\/\" \/>\n<meta property=\"og:site_name\" content=\"Lop12.edu.vn - C\u1ed9ng \u0111\u1ed3ng h\u1ecdc sinh l\u1edbp 12 l\u1edbn nh\u1ea5t t\u1ea1i Vi\u1ec7t Nam\" \/>\n<meta property=\"article:published_time\" content=\"2019-10-13T04:58:07+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2019-10-13T04:58:08+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/lop12.edu.vn\/wp-content\/uploads\/2019\/10\/l15.png\" \/>\n\t<meta property=\"og:image:width\" content=\"696\" \/>\n\t<meta property=\"og:image:height\" content=\"385\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"author\" content=\"Nguy\u1ec5n M\u01a1\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Nguy\u1ec5n M\u01a1\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/lop12.edu.vn\/ly-thuyet-sinh-hoc-10-bai-15-thuc-hanh-mot-so-thi-nghiem-ve-enzim\/\",\"url\":\"https:\/\/lop12.edu.vn\/ly-thuyet-sinh-hoc-10-bai-15-thuc-hanh-mot-so-thi-nghiem-ve-enzim\/\",\"name\":\"L\u00fd thuy\u1ebft Sinh h\u1ecdc 10 B\u00e0i 15: Th\u1ef1c h\u00e0nh: M\u1ed9t s\u1ed1 th\u00ed nghi\u1ec7m v\u1ec1 enzim\",\"isPartOf\":{\"@id\":\"https:\/\/lop12.edu.vn\/#website\"},\"datePublished\":\"2019-10-13T04:58:07+00:00\",\"dateModified\":\"2019-10-13T04:58:08+00:00\",\"author\":{\"@id\":\"https:\/\/lop12.edu.vn\/#\/schema\/person\/104e47bfb6189ee6fa1aa67e1f9107a2\"},\"breadcrumb\":{\"@id\":\"https:\/\/lop12.edu.vn\/ly-thuyet-sinh-hoc-10-bai-15-thuc-hanh-mot-so-thi-nghiem-ve-enzim\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/lop12.edu.vn\/ly-thuyet-sinh-hoc-10-bai-15-thuc-hanh-mot-so-thi-nghiem-ve-enzim\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/lop12.edu.vn\/ly-thuyet-sinh-hoc-10-bai-15-thuc-hanh-mot-so-thi-nghiem-ve-enzim\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/lop12.edu.vn\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"L\u00fd thuy\u1ebft Sinh h\u1ecdc 10 B\u00e0i 15: Th\u1ef1c h\u00e0nh: M\u1ed9t s\u1ed1 th\u00ed nghi\u1ec7m v\u1ec1 enzim\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/lop12.edu.vn\/#website\",\"url\":\"https:\/\/lop12.edu.vn\/\",\"name\":\"Lop12.edu.vn - C\u1ed9ng \u0111\u1ed3ng h\u1ecdc sinh l\u1edbp 12\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/lop12.edu.vn\/?s={search_term_string}\"},\"query-input\":\"required name=search_term_string\"}],\"inLanguage\":\"en-US\"},{\"@type\":\"Person\",\"@id\":\"https:\/\/lop12.edu.vn\/#\/schema\/person\/104e47bfb6189ee6fa1aa67e1f9107a2\",\"name\":\"Nguy\u1ec5n M\u01a1\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/lop12.edu.vn\/#\/schema\/person\/image\/\",\"url\":\"https:\/\/secure.gravatar.com\/avatar\/20d6905502209505aa7a21b55419ebe9?s=96&d=mm&r=g\",\"contentUrl\":\"https:\/\/secure.gravatar.com\/avatar\/20d6905502209505aa7a21b55419ebe9?s=96&d=mm&r=g\",\"caption\":\"Nguy\u1ec5n M\u01a1\"},\"url\":\"https:\/\/lop12.edu.vn\/author\/mont\/\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"L\u00fd thuy\u1ebft Sinh h\u1ecdc 10 B\u00e0i 15: Th\u1ef1c h\u00e0nh: M\u1ed9t s\u1ed1 th\u00ed nghi\u1ec7m v\u1ec1 enzim","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/lop12.edu.vn\/ly-thuyet-sinh-hoc-10-bai-15-thuc-hanh-mot-so-thi-nghiem-ve-enzim\/","og_locale":"en_US","og_type":"article","og_title":"L\u00fd thuy\u1ebft Sinh h\u1ecdc 10 B\u00e0i 15: Th\u1ef1c h\u00e0nh: M\u1ed9t s\u1ed1 th\u00ed nghi\u1ec7m v\u1ec1 enzim","og_description":"L\u00fd thuy\u1ebft Sinh h\u1ecdc 10 B\u00e0i 15: Th\u1ef1c h\u00e0nh: M\u1ed9t s\u1ed1 th\u00ed nghi\u1ec7m v\u1ec1 enzim I. 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